In Portal when you fight Glados, her lines during the fight really cracked me up, as well as her lines while you were trying to find her. Its a great game though so its really hard to pick a certain part.
The end of Beyond Good And Evil, another game I really loved and had a ton of favourite parts but jeezy chreezy that was the hardest boss fight I ever beat, so it left a good feeling of smug satisfaction. Granted, I hated the cliff hanger part, it was only last year I think that they announced that there would be more sequels.
I also love the desert temple in Ocarina Of Time and the Zora level in Majora's Mask, I just loved swimming around as a Zora. Final Fantasy IX has a ton of moments that i love so its too hard to pick just one.
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