What are you doing at this very moment?

[Beowulf]

Originally Posted by Duane

A tone like Angus Young's, well between his and Eddy Van Halens Brown Sound.

So your looking at a vintage Marshall amp sound, and a guitar with Gibson style P.A.F pickups (Angus Young uses a Gibson SG straight into a Marshall amp, and Eddie used a P.A.F pickup in his Frankenstein guitar allegedly into a modified Marshall amp at the time) ?

What Guitar, amp and effects do you use, and then I can give some suggestions ?

[Duane]

BC Rich Bronze Series warlock guitar and a Frontman 15 R guitar amp.

[Duane]

I'm thinking of upgrading to a Fender Strat. They better than this piece of shit.

[Beneath the Shadows]

I'm trying to get my youngest son to fall asleep.

[Beowulf]

Originally Posted by Duane

I'm thinking of upgrading to a Fender Strat. They better than this piece of shit.

If your looking for the Eddie Van Halen sound in a strat, you will need a Strat with a Humbucking pick up in the bridge (Eddie used a `59 P.A.F pick up from a Gibson guitar in his orignal Frankenstrat, but a Seymour Duncan JB pick up in the bridge will do fine for the sounds your looking for).

One such model of Fender is the `American Deluxe Stratocaster® QMT HSS`

Fender `American Deluxe Stratocaster® QMT HSS`


Or have you considered the Ibanez RG range which are both incredibly versitile instruments with fantastic playability and sound, but are also excellent value for money !!!.

Click here for Ibanez RG Series


I hope this helps, if you have any questions feel free to PM me !!!.

[Albert Mond]

I'm on the internets.

[All Shall Be Judged]

The Judge has the place to herself for a couple of hours, having sent her accomplice in world domination to the pub to watch the Arsenal match. She is currently listening to The Wall and whiling away an idle hour on the internet. Soon she shall touch up her hair colour, clean the bathroom, and raid JSTOR while writing about William Burroughs.

[Albert Mond]

Originally Posted by All Shall Be Judged

The Wall

Win.
epicblacktext

[All Shall Be Judged]

Originally Posted by Albert Mond

Win.
epicblacktext

The Wall is excellent, although I'm currently lamenting my lack of something to smoke.

[Alarica]

Feeling rather old. Have barely drunk alcohol in the last year, and got bladdered last night, and hadnt eaten. Christ I'm paying for it today.

[Albert Mond]

Titre du document / Document title
Construction of a restriction-endonuclease-Eam I05I-generated T-vector for high-throughput cloning and expression
Auteur(s) / Author(s)
BAOLI WANG (1) ; HUI LIANG (2) ; RUI LIU (1) ; XIAOXIA LI (1 3) ; BEI SUN (1) ; RUI ZHANG (1) ; SHANYI GUO (1) ; GANG GUO (1) ; JINGYU ZHANG (1) ; CHENLIN DAI (4) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Key Research Laboratory of Hormone and Development Affiliated to the Ministry of Health, Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, CHINE
(2) Department of Medical Diagnosis, Tianjin Medical University, Tianjin 300070, CHINE
(3) College of Basic Medical Science, Tianjin Medical University, Tianjin 300070, CHINE
(4) Tianjin Medical University Hospital, Tianjin 300070, CHINE

Résumé / Abstract
A novel T-vector was constructed that could be used for direct cloning and expression of PCR-amplified cDNA. The technique was based on the insertion into the parent vector of two endonuclease-Eam I 1051 restriction sequences spaced by an expression cassette of the full-length β-galactosidase, which helped to improve cloning efficiency and to minimize the non-recombinant background of the T-vector when used to clone PCR products. Moreover, this method took advantage of the reconstitution of the rarest restriction sequence of Mssl to enable directional cloning. These advantages make the T-vector suitable for high-throughput expression and analysis.
Revue / Journal Title
Biotechnology and applied biochemistry ISSN 0885-4513 CODEN BABIEC
Source / Source
2007, vol. 48 (1), pp. 29-33 [5 page(s) (article)] (12 ref.)
Langue / Language
Anglais

Editeur / Publisher
Portland Press, London, ROYAUME-UNI (1986) (Revue)

Mots-clés anglais / English Keywords
Hydrolases ; Glycosidases ; O-Glycosidases ; Enzyme ; Polymerase chain reaction ; β-Galactosidase ; Vector ; Restriction endonuclease ;
Mots-clés français / French Keywords
Hydrolases ; Glycosidases ; O-Glycosidases ; Enzyme ; Réaction chaîne polymérase ; β-Galactosidase ; Vecteur ; Restriction endonuclease ;
Mots-clés espagnols / Spanish Keywords
Hydrolases ; Glycosidases ; O-Glycosidases ; Enzima ; Reacción cadena polimerasa ; β-Galactosidase ; Vector ; Restriction endonuclease ;
Mots-clés d'auteur / Author Keywords
directional cloning ; β-galactosidase; high-throughput expression ; case chain reaction (PCR) ; T-vector. polymerase chain reaction (PCR) ; T-vector ;
Localisation / Location
INIST-CNRS, Cote INIST : 17979, 35400016082084.0040

[Beowulf]

Checking my messages and wondering what to listen to !!!.

[Desdemona]

Feeling tired, browsing Gnet and on MSN

[Beowulf]

Working on Photoshop and listening to music !!!.

[Alarica]

Feeling both proud and angry with my 8yr old, and yet cant tell her I'm proud of her actions. But I wish I'd had her courage when I was her age!

[Duane]

I'm learning German, it's a really fun language.

[Geoluhread]

eating carrots while talking to my boyfriend.

[Beowulf]

Reading and listening to the radio !!!.

[kikrox]

taking it in the ass.

[Beowulf]

Listening to Nocturnus and doing some work in Photoshop !!!.

[RavenHallows.]

Being utterly and completely bored, to be honest. Listenign to a great band (Die Sektor) though. My dear mother just started asking me about the safety of this here forum.. Cookies, anyone?

[Nexus-6]

Playing with the stitches in my mouth.

[Raivyn]

Listening to the bf ramble on about different job opportunities in Florida.. Planning our upcoming move. Sipping coffee and eating these awesome white chocolate chunk cookies.

[Beowulf]

Chatting to Geo and listening to Planet Rock on the radio (Digital Radio station) !!!.

http://www.planetrock.co.uk/

[Delkaetre]

Sitting in my underwear, listening to Christian Death, talking to my Gent, being slightly intimidated by his brother's girlfriend (though perhaps not for reasons you might expect), and considering going to bed, as it's 1am here.